vibrio alginolyticus pronunciation

Quite strikingly, the peroxisomal and apoptotic pathways are activated by V. alginolyticusWT infection, whereas phagocytosis and cell adhesion were enhanced in V. alginolyticusvscC infection. Nevertheless, the proportions of apoptotic cells (early apoptosis rate + late apoptosis rate) varied considerably in relation to the H2O2 concentration applied. Mustapha S, Mustapha EM, Nozha C. Vibrio Alginolyticus: an emerging pathogen of foodborne diseases. V. alginolyticus would later be isolated in 1997, as a result of a pathogenic outbreak in Russia.9, 12, V. alginolyticus can be comma or coccoid shaped and is Gram negative.7 There are a few different strains, many of which are pathogenic. The resultant fragment lacked all coding sequences of the vscC gene and was inserted in the suicide vector pDM4 to generate pDM4/vscC. 2003;41(7):3427-3429. Rate the pronunciation difficulty of vibrio alginolyticus. Identification and Characterization of a Novel Type III Secretion System in Trh-Positive Vibrio Parahaemolyticus Strain TH3996 Reveal Genetic Lineage and Diversity of Pathogenic Machinery Beyond the Species Level. Next, we assessed the levels of H2O2, a common ROS downstream of O2, induced by Vibrio challenges (3335). doi:10.1016/j.dci.2017.07.012, 22. Outer Membrane Protein A (OmpA) Is a Potential Virulence Factor of Vibrio Alginolyticus Strains Isolated From Diseased Fish. A case of vibriosis in Asian seabass reared in open sea floating cages is reported. Pronunciation of vibrio alginolyticus with 1 audio pronunciation, 2 translations and more for vibrio alginolyticus. 2006;69A:260265. doi:10.1590/0001-3765201820170730, 33. Perez-Riverol Y, Csordas A, Bai J, Bernal-Llinares M, Hewapathirana S, Kundu DJ, et al. 19458. Here, p53 target protein was significantly upregulated by either V. alginolyticusWT infection or H2O2 treatment, rather than V. alginolyticusvscC infection, strongly suggesting that virulent Vibrio infection or ROS boost could activate p53 signaling pathways. Vibrio alginolyticus is a Gram-negative bacterium ubiquitously found in aquatic and marine habitats and poses considerable health risks to marine animals and humans alike via the local food-chains (1). Vibrio alginolyticus Extracellular Superoxide Dismutase in Biology and Medicine. Hemocytes after H2O2 stimulation and Chp53-target protein-depletion were applied for total RNA extraction by using TRIzol reagent (Invitrogen). Data Definition and Standards. 1961 Beneckea alginolytica (Miyamoto et al. Raw LC-MS/MS data were converted to MGF files by using Proteome Discoverer (Thermo scientific). doi:10.1159/000158843, 71. Statistical analysis was performed with FlowJo (v10.0 software). On the other hand, phagocytosis- and cell adhesion-associated pathways such as cell adhesion molecules, endocytosis, and phagosomes were enhanced in the V. alginolyticusvscC challenged group. PLoS ONE. doi:10.1016/j.aquaculture.2007.04.074, 81. A volcano-gram was drawn to filter differentially expressed proteins (DEPs) between the PBS-, V. alginolyticusWT-, and V. alginolyticusvscC-injected groups. The x-axis displays time lapsed post-injection, and the y-axis shows bacterial clearance efficiency (recovered bacteria). Pronunciation of the word(s) "Vibrio Alginolyticus".Please subscribe to my Channel if you found these pronunciation videos useful. Water (and its associated microorganisms, animals, and plants) is the natural habitat of most vibrios. Next, another 400 l binding buffer was added to the suspension, before analysis by a Guava easyCyteTM flow cytometer (Millipore). Pasare C, Medzhitov R. Toll-Like Receptors: Linking Innate and Adaptive Immunity. (A) Distribution of enriched GO terms among three clusters (common DEPs, V. alginolyticusWT-specific DEPs, and V. alginolyticusvscC-specific DEPs). After all, we observed no significant change in intracellular H2O2 level, even the apoptotic levels after V. alginolyticusvscC strain infection. Here, we provided the first direct and specific evidence on the molecular basis for underlying virulence-related immunomodulation of mollusk host response during infection by V. alginolyticus. Vibrio alginolyticus are commonly found in aquatic environments. Analysis on hemocyte apoptosis shows that H2O2 treatment could cause a marked increase in apoptotic cells relative to the control group. doi:10.1006/meth.2001.1262, 30. Register Solid lines, regulatory mechanisms experimentally verified in this study; dotted lines, conjectural conclusions from the proteomic data. Basic Sci Rep (1999) 99(22):293441. Vibrio alginolyticus Triggers Inflammatory Response in Mouse proposed a new name for the biotype species: Vibrio alginolyticus. Cancer Lett (2016) 370(2):27985. In applied contexts, activation of TLR6 following V. alginolyticusvscC strain infection in oyster hemocytes carries the implications of potential use of attenuated strains in host immune priming in an immunologically mild manner. Then, cells were subjected to repeated freeze-thaw cycles in liquid nitrogen until all cells were lysed. Nat News views feature (2000) 408:30710. Acad Bras Cienc (2018) 90(4):340314. Smolikova LM, Lomov LM, Khomenko TV, Murnachev GP, Kudriakova TA, Fetsailova OP, Sanamiants EM, Makedonova LD, Kachkina GV, Data were analyzed in GraphPad 8.0 by two-way ANOVA and presented as mean SEM (n = 3). 2013;2(4):302-309. The results suggest that apoptosis rate increased significantly (p<0.05) after challenge with the V. alginolyticusWT strain (dsGFP + V. alginolyticusWT strain), by 1.6-fold relative to the control group (dsGFP + PBS). PRIDE Inspector Toolsuite: Moving Toward a Universal Visualization Tool for Proteomics Data Standard Formats and Quality Assessment of ProteomeXchange Datasets. 4. Collectively, we conclude that adaptive responses in host defenses are partially determined by pathogen virulence, in order to safeguard efficiency and timeliness in bacterial clearance. Front Cell Infect Microbiol (2020) 10:301. doi:10.3389/fcimb.2020.00301, 13. Statistical significance was determined at *p < 0.05 and **p < 0.01. Julia. doi:10.1128/genomeA.00912-16. Heatmaps were organized to display the expression level of DEPs in each group by TBtools software (27). Bonekamp NA, Volkl A, Fahimi HD, Schrader M. Reactive Oxygen Species and Peroxisomes: Struggling for Balance. Mol Plant (2020) 13(8):1194202. Deng Y, Chen C, Zhao Z, Zhao J, Jacq A, Huang X, Yany Y. Perez-Riverol Y, Xu QW, Wang R, Uszkoreit J, Griss J, Sanchez A, et al. Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio alginolyticus are the three most common species that cause illness in the United States, reports the CDC. doi:10.1038/s41598-020-63676-3, 82. Front Microbiol (2017) 8:86. doi:10.3389/fmicb.2017.00086, 42. Lacroix M, Riscal R, Arena G, Linares LK, Le Cam L. Metabolic Functions of the Tumor Suppressor P53: Implications in Normal Physiology, Metabolic Disorders, and Cancer. J Bot (2012) 2012:217037. doi:10.1155/2012/217037, 34. Mar Environ Res (2020) 154:1048709. The y-axis shows the -log10 (p-values), and the x-axis shows the difference in expression as measured in log2 (fold change). Congrats! Vibrio Species Causing Vibriosis | Vibrio Illness (Vibriosis) | CDC At least 10,000 cell events were gated. In oyster, the significant decrease of sGC in V. alginolyticusvscC infection group could balance the NO binding-induced activation of sGC. Define halophilic. Three oysters were pooled as one sample, and three samples were collected at each time point. Zhang G, Li L, Meng J, Qi H, Qu T, Xu F, et al. As a marine invertebrate model ideal for studying the interplay between environment, microbial pathogens, and host in disease dynamics (89), the oyster is useful for investigating molecular interactions between pathogenic bacteria and host cells, which in turn shed light on potential determinants of infection outcomes in metazoan hosts. Vibrio alginolyticus | definition of Vibrio alginolyticus by Mol Metab (2020) 33:222. Viruses (2018) 10(3):13344. (A) Workflow of the construction of attenuated alginolyticusvscC strain. Synthetic Toll-Like Receptor Agonists for the Development of Powerful Malaria Vaccines: A Patent Review. Antonie van Leeuwenhoek (2011) 100(2):291307. For false discovery rate (FDR) calculations, an automatic decoy database search strategy was employed to estimate FDR by using a Prcolator algorithm. Microbiol (Reading) (2010) 156(Pt 9):286472. Bangladesh J Microbiol. Forty oysters were randomly assigned into four groups and placed into four tanks: dsGFP + PBS, dsGFP + V. alginolyticusWT, dsp53tp + PBS, dsp53tp + V. alginolyticusWT. 1971 Pseudomonas creosotensis O'Neill et al. [4] V. alginolyticus is rare cause of bacteremia in immunocompromised hosts. It also seems reasonable to assume that TLR6 becomes activated during infection by the V. alginolyticusvscC strain. Subsequently, exogenously applied cGMP was injected into the oysters via the adductor muscle to test whether excess cGMP levels would cause oyster hemocyte apoptosis. V. alginolyticusvscC infection leads to attenuation of cellular defense of the host, along with lower levels of apoptosis. Nitric Oxide and Apoptosis Induced in Peyers Patches by Attenuated Strains of Salmonella Enterica Serovar Enteritidis. In our proteome data, peroxisome, lysosome, and apoptosis pathway were enriched specifically during virulent strain infection. Shazib Pervaizc. Mello DF, Trevisan R, Danielli NM, Dafre AL. In 1968, Sakazaki et al. Studies on halophilic vibrios causing a food poisoning outbreak in the city of Vladivostok. Finally, p53tp identified here was verified via RNA interference to regulate intrinsic apoptosis. Las especies que ms comnmente causan enfermedad en los seres humanos en los Estados Unidos son Vibrio parahaemolyticus, Vibrio vulnificus y Vibrio alginolyticus. Vibrio alginolyticus Vibrios also cause wound and soft-tissue infections among persons who have contact with water; for example, This assay evaluated by experimental infection the pathogenicity of, Dictionary, Encyclopedia and Thesaurus - The Free Dictionary, the webmaster's page for free fun content, Bacteria Recovered from Cultured Gilt-Head Seabream (Sparus aurata) and their Antimicrobial Susceptibilities, Effect Of carbon sources on bacillus sp.R2 chitinase production, Aislamiento y caracterizacion del bacteriofago Va1 especifico a Vibrio alginolyticus, Anti-idiotypic monoclonal antibodies specific for immunoglobulins against the lipopolysaccharide antigen of Brucella, Flagellum failure lets bacteria steer: buckling of appendage drives tiny two-point turn, Incidence and trends of infection with pathogens transmitted commonly through food--Foodborne Diseases Active Surveillance Network, 10 U.S. sites, 1996-2012, Probiotics as antiviral agents in shrimp aquaculture, Identificacion de bacterias del genero vibrio asociadas a zonas productoras de moluscos bivalvos, estado Sucre, Venezuela, Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (decapoda: penaeidae), Novel Mycobacterium species in seahorses with tail rot, Isolation and experimental infection with Vibrio alginolyticus in the sea horse, Hippocampus reidi Ginsburg, 1933 (Osteichthyes: Syngnathidae) in Brazil/Isolamento e infeccao experimental de Vibrio alginolyticus em cavalo-marinho, Hippocampus reidi Ginsburg, 1933 (Osteichthyes: Syngnathidae) no Brasil, Vibrio Parahaemolyticus Sodium/Glucose Transporter. Taken as a whole, our data support the notion that elevations in intracellular cGMP levels elicit hemocytes apoptosis, as in the contexts of virulent Vibrio infection. Zhang Y, He X, Yu F, Yu Z. doi:10.1128/IAI.01184-08, 27. Hence, 0.1 to 1 mM H2O2 was applied as a treatment for analysis on apoptotic events in hemocytes and their gene expression patterns of p53 target protein (p53tp). doi:10.1016/j.redox.2020.101508, 45. The attenuated strain V. alginolyticusvscC was constructed by mutation of vscC fragment in the V. alginolyticusWT genome, which constitutes part of the core components of T3SS (26). When external microorganisms launch invasion, oyster hemocytes respond by producing and leveraging a range of toxic metabolites (such as ROS, nitric oxide, etc.) The Molecular Mechanism Underlying Pro-Apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 in Crassostrea Hongkongensis. In the last decade, an important increase in the frequency of V. alginolyticus isolated from cultured diseased marine fish had been recorded (Balebona et al. US English. https://www.definitions.net/pronounce/vibrio+alginolyticus. and many lysosomal enzymes (7981) for defense, while apoptosis is induced as a hemocyte elimination process to clear spent or infected cells. In addition, 17 upregulated and 10 downregulated proteins were found to be differentially expressed in both cases of oysters injected with V. alginolyticusWT strain and V. alginolyticusvscC. Briefly, cell lysates were loaded into microplates followed by addition of 50 l HRP-conjugated reagent, covering the plates with a plate sealer and incubating the samples at 37C for 60 min. Q4: The lower left quadrant (PI and annexin V negative cells) shows living cell population. In a Coastal Transect: Occurrence and Multiple Virulence Factors. Our findings suggest that the oyster immune system compute decisions on mild to severe responses against differentially virulent Vibrio strains (Figure7). Q3: On the lower right quadrant were cells stained as annexin V-FITC positive only, corresponding to cells undergoing early apoptosis. Immunofluorescent staining was carried out to confirm the loss of protein secretion function in the mutant strain (V. alginolyticusvscC). Human infections by V. alginolyticus occur primarily through consumption of insufficiently cooked aquatic food products, with consequences such as diarrhea, gastroenteritis, and septicemia (2). 7. lbertini MC, Accorsi A, Teodori L, Pierfelici L, Uguccioni F, Rocchi MBL, Burattini S, Citterio B. Vibrio: What you should know about the deadly bacteria | CNN To elucidate the molecular mechanisms underlying host immune defenses against Vibrio strains with distinct virulence profiles, GO term enrichment was performed to annotate and compare pathways induced in oysters injected with V. alginolyticusWT and V. alginolyticusvscC. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was performed to analyze cell viability and determine the appropriate H2O2 concentrations for hemocytes treatment, according to previous reports (28). Mao F, Wong NK, Lin Y, Zhang X, Liu K, Huang M, et al. In this light, the construction of knockout mutant strains such as HY9901tyeA and clpP provided evidence for attenuated live vaccines in fish and shrimp (15, 16). In May 2023, Frontiers adopted a new reporting platform to be Counter 5 compliant, in line with industry standards. Hence, we designed RNA interference assay to investigate the regulatory role of Chp53tp during V. alginolyticusWT infection. Moreover, our work is consistent with previous findings that implicate activation of intracellular cGMP as an important event in cell apoptosis (7678). ACS Chem Neurosci (2017) 8(7):143847. SX provided the experimental animals. Hemolymph were collected from the PBS-, V. alginolyticusWT-, and V. alginolyticusvscC-injected groups for protein extraction. Vibrio alginolyticus definition: any curved or spiral rodlike Gram-negative bacterium of the genus Vibrio , including V. | Meaning, pronunciation, translations and Figure2 Differentially expressed proteins after injection of the V. alginolyticusWT strain or V. alginolyticusvscC strain. John W, Denninger MAM. At 100 mM cGMP, apoptosis rate increased from 16.58% (early apoptosis, 9.70%; late apoptosis, 6.87%) to 41.16% (early apoptosis, 29.03%; late apoptosis, 12.13%) (Figure6B). 2012;29(1):1-6. Curr Vasc Pharmacol (2005) 3:2219. Vibrio alginolyticus - an overview | ScienceDirect Topics [2] S.I. US English. Gu D, Zhang J, Hao Y, Xu R, Zhang Y, Ma Y, et al. To view the purposes they believe they have legitimate interest for, or to object to this data processing use the vendor list link below. Comparative Transcriptomic Analysis of Surf Clams (Paphia Undulate) Infected With Two Strains of Vibrio Spp. [4] Cold Spring Harb Protoc (2018) 2018(6):14. doi:10.1093/nar/gkaa666, 55. (A) Volcano plots show the relationship between fold-changes and significance for the V. alginolyticusWT (left panel) and V. alginolyticusvscC (right panel) challenged groups, compared with the uninfected control. Peptide samples were collected at 214 nm at every min followed by freeze-drying. Data were analyzed by two-way ANOVA and presented as mean SEM (n=3); ns, no significance; **p < 0.01; ***p < 0.001. Recent Advances in Toll Like Receptor-Targeting Glycoconjugate Vaccines. doi:10.2741/3228, 73. An Innovative Method for Rapid Identification and Detection of Aproximadamente una docena de especies de Vibrio pueden causar enfermedad en los seres humanos, la cual se conoce como vibriosis. Protein expression levels were normalized by z-score normalization method. Lake Superior State University, United States, Institute of Oceanology, Chinese Academy of Sciences (CAS), China. doi:10.1016/j.molmet.2019.10.002, 57. Three oysters were pooled as one sample, and three biological samples were collected. Remarkably, p53tp levels were found to be elevated following V. alginolyticusWT strain injection or H2O2 treatment. (C) Histogram displays the statistical analysis on apoptotic cells under different treatments. To elucidate molecular mechanisms underlying the infection of Vibrio spp. Data were analyzed by one-way ANOVA and presented as mean SEM (n=3); *p < 0.05; **p < 0.01. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method. Robinson AN, Green TJ. [4] It occasionally causes eye, ear, and wound infections. in 1965 the second biotype be moved into its own species. doi: 10.1002/(SICI)1098-1136(19990115)25:2<154::AID-GLIA6>3.0.CO;2-S, 38. Briefly, 2 mg of IBT reagent was dissolved in 80 l isopropanol. Siddiqui1 R, Alam MM, Naser MN, Otomo Y, Yasmin M, Nessa J, Ahsan CR. doi:10.1016/s0891-5849(03)00275-2, 44. Proc Natl Acad Sci U S A (2019) 116(28):1423847. The most common species causing vibriosis in the United States are Vibrio parahaemolyticus, Vibrio The expression levels of p53 target protein rose significantly in response to H2O2 (in the range of 0.1 to 1 mM), supporting the involvement of p53-dependent apoptotic pathways in H2O2-induced cell apoptosis (Figure4E). Accordingly, we also provide evidence that intracellular cGMP elevations were only induced after V. alginolyticusWT challenge, whereas cGMP content was largely unaltered following infection by the V. alginolyticusvscC strain. I. Etiology and Epizootiology. Two-year-old C. hongkongensis (shell length 100 10 mm) were collected from Zhanjiang, Guangdong Province, China, and acclimated to laboratory conditions by rearing in aerated sand-filtered seawater at ambient temperature (27 1C) and appropriate salinity (25) for 2 weeks prior to experiments. Domain prediction indicated a conserved LIFAT domain in the p53tp amino acid sequence as shown in Supplementary Figure4. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Biochim Biophys Acta (1999) 1411:33450. An In Vitro Co-Culture Mouse Model Demonstrates Efficient Vaccine-Mediated Control of Francisella Tularensis SCHU S4 and Identifies Nitric Oxide as a Predictor of Efficacy. V. parahaemolyticus historically had included two biotypes, but it was suggested by Zen-Yoji et al. Zhao Z, Liu J, Deng Y, Huang W, Ren C, Call DR, et al. doi: 10.1016/S0014-5793(98)01410-0, 46. Fish Shellfish Immunol (2020) 98:91722. For its relatively high pathogenic potential, the need to develop vaccines, which immunologically target this pathogen in prophylactic applications, is particularly urgent. Following challenge with V. alginolyticusWT strain + dsGFP, expression levels of Chp53 target protein were markedly upregulated by 2.4 folds (p<0.0001). Dang X, Wong NK, Xie Y, Thiyagarajan V, Mao F, Zhang X, et al. Crowdsourced audio pronunciation dictionary for 89 languages, with meanings, synonyms, sentence usages, translations and much more. Previous research has also provided evidence for that TNF expression level inhibited by blocking TLRs signal in oysters when Vibrio parahaemolyticus invaded (87). 1 Jul 2023. Subsequently, hemolymph was lysed in 200 l cold PBS containing 0.05% Triton X-100 for 1 h. Lysates from each tube (10 l) were serially diluted in PBS, from which 100 l lysates was spread on LB plates followed by overnight culture for enumeration of bacterial colony counts to determine bacterial clearance efficiency. Fattman CL, Schaefer LM, Oury TD. Vibrio alginolyticus is a food-borne marine Vibrio that causes gastroenteritis, otitis media, otitis externa, and septicemia in humans. 1961, Vibrio alginolyticus is a Gram-negative marine bacterium. Vibrio alginolyticus is a food-borne marine Vibrio that causes gastroenteritis, otitis media, otitis externa, and septicemia in humans. 1. Chen L, Liu S, Tao Y. Copyright 2021 Mao, Liu, Wong, Zhang, Yi, Xiang, Xiao, Yu and Zhang. Intracellular cGMP concentrations were calculated via a standard curve as shown in Supplementary Figure6. Previous studies reported that apoptosis induced by H2O2 is regulated through control of O2 concentrations (45), with particular implications for respiratory bursts-dependent apoptosis of oyster hemocytes following virulent bacterial infection (4648). Cai YM, Zhang YD, Yang L. NO Donors and NO Delivery Methods for Controlling Biofilms in Chronic Lung Infections. We believe that attenuated pathogen strains such as that of virulent Vibrio species in applications deserve more attention, as immune priming in the oyster has been launched during the last few years for exploration of immunity system evolution to prevent disease outbreaks (8993). Listen to the audio pronunciation of Vibrio alginolyticus on pronouncekiwi The precipitates were redissolved in a lysis buffer and prepared for quantification by using the Bradford method. Sammons MA, Nguyen TT, McDade SS, Fischer M. Tumor Suppressor P53: From Engaging DNA to Target Gene Regulation. As a commercially valuable species, it is endemic to the estuarine and coastal regions of the South China Sea, with an aquaculture history of more than 700 years (17). WebGI, wound infections, septicemia. Blood Vessels (1991) 28(1-3):5261. The plates were processed to remove residual liquid in the wells after wash step. Subsequently, we found some activated pathways shared between the virulent and attenuated strains during Vibrio challenge, including PI3K-Akt signaling pathway, calcium signaling pathway, ECM-receptor interaction, and cell adhesion molecules. David. Vibrio pathogenicity is a complex interaction of abiotic and biotic factors, including high temperatures [23, 24] low salinities , host and bacterial genotypes [26, 27]. This page was last edited on 31 March 2017, at 21:13.
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